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簡要描述：CRL-9520 SV-HUC-1 人膀胱上皮永生化細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，提供的細胞株背景清楚，提供參考文獻和培養(yǎng)條件！
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CRL-9520 SV-HUC-1 人膀胱上皮永生化細胞
ATCC® Number: CRL-9520™ Price: $417.00
Designations: SV-HUC-1
Depositors: Wisconsin Alumni Res. Fndn.
Biosafety Level: 2 [CELLS CONTAIN ADENOVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens （human）
Morphology: epithelial
CRL-9520 SV-HUC-1 人膀胱上皮永生化細胞
Source: Organ: ureter
Tissue: uroepithelium
Cell Type: epithelial SV40 immortalizedSV40 transformed
Cellular Products: uroepithelial keratins; SV40 T antigen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
Cytogenetic Analysis: The chromosome number distribution is bimodal with 50% of the cells near diploid （mode = 44 chromosomes） and 50% tetraploid （mode = 88）. Both populations consistently showed seven marker chromosomes: 5p+, del（6）（p11）, 9q+, 11p+, 15q-, 19p+ and Xp+. Most cells lacked a normal chromosome 15. Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21, while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation.
Age: 11 years adult
Gender: male
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% Trypsin, 0.03% EDTA solution and allow the flask to sit at room temperature for 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subc*tion Ratio: 1:2 to 1:5
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2004
recommended serum:ATCC 30-2020
derivative:ATCC CRL-9519
References: 3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990
CRL-9520 SV-HUC-1
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